Ces are listed in Table S1.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSupplementary MaterialRefer to Internet version on PubMed Central for supplementary material.AcknowledgmentsWe thank Steve Hahn, Stephen Tapscott, Barbara Wakimoto, Sue Biggins, J. Rodriguez, N. Bogenschutz, T. Cunningham, L. Lee, J. McKnight, L. Haselden, E. Alcid and the Biggns lab members for helpful discussions and ideas. This study was supported by NIH grant RO1 GM058465 to T.T. and by RO1 GM039484 to M.H. A.N.Y. was supported by Developmental Biology Predoctoral Coaching Grant T32HD007183 in the National Institutes of Child Health and Human Development.Mol Cell. Author manuscript; available in PMC 2014 April 11.Yadon et al.Page
Teruel et al. Journal of Biomedical Science 2013, 20:29 http://jbiomedsci/content/20/1/RESEARCHOpen AccessControl of post-translational modifications in antithrombin during murine post-natal improvement by miR-200aRa Teruel, Irene Mart ez-Mart ez, Jos?A Guerrero, Roc Gonz ez-Conejero, Mar E de la Morena-Barrio, Salam Salloum-Asfar, Ana B Arroyo, Sonia uila, Nuria Garc -Barber? Antonia Mi no, Vicente Vicente, Javier Corral and Constantino Mart ez*AbstractBackground: Developmental haemostatic research may possibly support identifying new components involved inside the manage of crucial haemostatic proteins like antithrombin, essentially the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content material in neonatal antithrombin compared with adult antithrombin in mice.2222867-16-3 web mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, had been 85 decrease in neonates in comparison with adults. In silico evaluation of miRNAs overexpressed in neonates revealed that mir-200a could possibly target these sialyltransferases. Moreover, in vitro studies in murine key hepatocytes sustain this prospective handle. Conclusions: These information recommend that as well as the direct protein regulation, microRNAs may perhaps also modulate qualitative traits of selected proteins by an indirect control of post-translational processes. Keyword phrases: miRNAs, Sialytransferases, Antithrombin, Post-translational modifications, Microarray, Post-natal developmentBackground MicroRNAs (miRNAs) are tiny non-coding RNAs implicated in the modulation of a big variety of physiological and pathological processes [1,2] via a mechanism based around the repression of protein translation or degradation of messenger RNAs [3].Price of 4-Bromo-2-ethylpyridine MiRNAs have been not too long ago involved in the modulation of several haemostatic aspects for example fibrinogen, tissue issue, and proteins implicated in platelet function [4-6].PMID:35227773 In fact, miRNAs may also be involved within the quantitative variations of components on the haemostatic program observed for the duration of improvement [7]. In humans, levels of haemostatic variables go in constant boost immediately after birth until reaching levels equivalent to these located in adults inside the very first year [8-10]. In particular, antithrombin, an anticoagulant serpin essential inside the handle on the hemostatic program [11], is substantially lowered (50 ) in plasma of neonates in comparison with adults* Correspondence: [email protected] Centro Regional de Hemodonaci , University of Murcia, IMIB, Spain, C/Ronda de Garay S/N, 30003, Murcia, Spain[8]. Regardless of these variations, neonates maintain a perfect haemostatic equilibrium. Accordingly, a developmental model amongst neonatal and adult period is excellent to study the mechanisms that re.