By gavage (2, 17). The 12th group of animals was the sham group which was not provided D-galactose. Immediately after two weeks, the 11 groups which had been given D-galactose had been randomly divided into aging control group (500 mg/kg D-galactose per 1 ml drinking water, for six weeks), optimistic manage group (500 mg/kg D-galactose per 1ml drinking water plus vitamin E 200 mg/kg/day by gavage for four weeks) and herb-treated groups like 9 groups that every single received 500 mg/kg D-galactose per 1 ml drinking water plus Z. officinale (250 mg/kg/day), G. glabra (150 mg/kg/day), R. officinalis (300 mg/kg/day), P. harmala (50 mg/kg/day), A. vera (150 mg/kg/day), S. hortensis (200 mg/kg/day), T. scordium (200 mg/kg/day), H. perforatum (Materials and MethodsChemicals Thiobarbituric acid (TBA), trichloroacetic acid (TCA), n-butanol, hexadecyltrimethyl ammonium bromide (HETAB), tri (2-pyridyl)-s-triazine (TPTZ), HCl, malondialdehyde (MDA), ferric chloride (FeCl36H2O), D-galactose, and vitamin E (Trolox) have been purchased from Merck (Germany).2-Bromo-4-chloro-3-fluorobenzaldehyde supplier Rat specific tumor necrosis factor- (TNF-), interlukine-1 (IL), interlukine-6 (IL-6), NF-kappa B (NF-b) ELISA kits have been purchased from BenderMed Systems (Austria). Testosterone and dehydroepiandrosterone ELISA kits had been bought from Dia Metra (Italy). Preparation of herbs, extraction, and lyophilization Herbs were supplied from the Research Institute of Medicinal Plants Karaj in the course of June 2009 and had been air-dried at room temperature. Samples were authenticated by a botanist (Y. Ajani), and voucher specimens have been preserved in the central herbarium of medicinal plants (RIMP). The scientific names and tested components on the herbal components are detailed in Table 1. The dried plants powder (40 g) wasTable 1. The scientific names and tested parts with the plant supplies Scientific name Zingiber officinale Glycyrrhiza glabra Rosmarinus officinalis Peganum harmala Aloe vera Satureja hortensis Teucrium scordium Hypericum perforatum Silybum marianum Tested components Rhizome Root Aerial components Seed Gel Aerial parts Aerial components Aerial components SeedExtraction yield (mg/g) 140.57 129.52 236.51 169.25 four.87 134 205 100.58 123.Used Dose (mg/kg) 250 150 300 50 150 200 200 135References 18 19 20 21 22 23 24 25Iran J Simple Med Sci, Vol. 16, No. 11, NovAnti-Aging Effects of Some Iranian Folk Medicinal HerbsMohammadirad A et almg/kg/day) and S. marianum (150 mg/kg/day), respectively by gavage for 4 weeks (18-26). Twenty-four hours after the last treatment, blood samples have been taken of every single animal beneath anesthesia through the tail vein. Serum samples were obtained by centrifuging the entire blood at 1000 ?g at 4 for 10 min and also the supernatants have been transferred into a number of microtubes for separate biochemical assays and maintained at -80 until the analyses had been performed.Formula of 37091-73-9 Biochemical markers including TNF-, IL-, IL-6, NF-b, ferric reducing total antioxidant power (TAP), lipid peroxidation (LPO) and male sex hormones like testosterone and dehydroepiandrosterone-sulfate (DHEA-S) had been measured in the serum.PMID:24101108 Measurement of LPO LPO was measured by the reaction of thiobarbituric acid (TBA) with lipid peroxides. Samples were mixed with TCA (20 ) and the precipitate was dispersed in H2SO4 (0.05 M). Immediately after addition of TBA (0.two in sodium sulfate), the sample was heated for 30 min in a boiling water bath. Then, TBA reactive substances (TBARS) as LPO marker adducts had been extracted by n-butanol and absorbance was measured at 532 nm as described in information in our prior wo.